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Optimizing light-sculpting microscopy for neuroscience

BioMedOptExp_website_fig_forRobertYesterday our most recent paper got published in Biomedical Optics Express. Our article, entitled “Optimizing and extending light-sculpting microscopy for fast functional imaging in neuroscience” studies various experimental trade-offs in temporal focusing based light-sculpting microscopy and shows how to optimize its performance based on specific experimental requirements. Furthermore, we show that synchronization of line-scanning techniques with rolling-shutter read-out of the scientific camera can reduce scattering effects and thus enhance image contrast at depth. Our theoretical and experimental results were corroborated by performing calcium-imaging of acute mouse brain slices expressing the calcium reporter GCaMP6m. In contrast to our previous work using wide-field TeFo, we were able to significantly increase the imaging field-of-view while maintaining physiologically relevant imaging speeds, even in the cases where a standard Ti:Sa laser was employed. We hope that these results will make light-sculpting a more practical and thus widely applicable imaging technique in the life sciences and neuroscience in particular.

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